Guinea-pig sympathetic postganglionic neurones contain haem oxygenase-2

Neuroreport. 1995 Dec 29;7(1):173-6.

Abstract

HAEM oxygenase-2 (HO-2) is the neuronal isoform of the only known mammalian enzyme generating the new transmitter candidate, carbon monoxide. Its distribution was investigated in the sympathetic nervous system. A 36 kDa HO-2 immunoreactive protein was identified in the particulate fraction of stellate ganglion and cerebellum in Western blots. Immunohistochemically, all noradrenergic and non-noradrenergic postganglionic neurones were HO-2 immunoreactive in prevertebral ganglia and cervical, thoracic, and lumbar sympathetic chain ganglia. Neither postganglionic nerve branches nor noradrenergic perivascular terminals were HO-2 immunoreactive under control conditions. However, accumulation of HO-2 immunoreactivity was found in noradrenergic axons in explanted sciatic nerves in which axonal transport was interrupted by crushing. We conclude that HO-2 is ubiquitous in perikarya of postganglionic sympathetic neurones, is subjected to axonal transport but does not accumulate at sympathetic neuroeffector junctions. This distribution favours a general role of HO-2 in sympathetic neuronal metabolism rather than a specific association with transmission at autonomic neuroeffector junctions.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Axons / enzymology
  • Carbon Monoxide / metabolism
  • Cricetinae
  • Heme Oxygenase (Decyclizing) / analysis*
  • Immunohistochemistry
  • Isoenzymes / analysis*
  • Molecular Weight
  • Neurons / enzymology*
  • Sympathetic Fibers, Postganglionic / cytology
  • Sympathetic Fibers, Postganglionic / enzymology*
  • Tyrosine 3-Monooxygenase / analysis

Substances

  • Isoenzymes
  • Carbon Monoxide
  • Heme Oxygenase (Decyclizing)
  • Tyrosine 3-Monooxygenase