A new generation of the IMAGIC image processing system

J Struct Biol. Jan-Feb 1996;116(1):17-24. doi: 10.1006/jsbi.1996.0004.


One of the aims of modern microscopy is to quantify two-, three-, or even four-dimensional phenomena in biology, medicine, and material sciences. The requirements imposed on software by such data processing are exemplified by the design considerations of the IMAGIC-5 software system. This system includes facilities for multivariate statistical analysis of large data sets, for correlation averaging of two-dimensional crystals, and for three-dimensional reconstruction of macromolecular structures. The molecules may be arranged as two-dimensional crystals, as helices, or as single particles with arbitrary pointgroup symmetry. IMAGIC's novel angular reconstitution approach allows for the rapid determination of three-dimensional structures of uncrystallized molecules to high resolution. The general organization, user interaction strategy, file structure, and extendibility of IMAGIC are discussed and illustrated with some practical examples.

Publication types

  • Research Support, Non-U.S. Gov't
  • Review

MeSH terms

  • Animals
  • Bacteriophages / ultrastructure
  • Computer Simulation*
  • Escherichia coli / ultrastructure
  • Hemoglobins / ultrastructure
  • Microscopy, Electron*
  • Multivariate Analysis
  • Ribosomes / ultrastructure
  • Snails
  • Software*
  • Viral Proteins / chemistry
  • Viral Proteins / ultrastructure


  • Hemoglobins
  • Viral Proteins