SPIDER and WEB: processing and visualization of images in 3D electron microscopy and related fields

J Struct Biol. 1996 Jan-Feb;116(1):190-9. doi: 10.1006/jsbi.1996.0030.


The SPIDER system has evolved into a comprehensive tool set for image processing, making use of modern graphics interfacing in the VMS and UNIX environment. SPIDER and WEB handle the complementary tasks of batch processing and visualization of the results. The emphasis of the SPIDER system remains in the area of single particle averaging and reconstruction, although a variety of other application areas have been added. Novel features are a suite of operations relating to the determination, modeling, and correction of the contrast transfer function and the availability of the entire documentation in hypertext format.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Antigen-Antibody Complex / ultrastructure
  • Calcium Channels / ultrastructure
  • Computer Graphics
  • Computer Simulation*
  • Hemocyanins / ultrastructure
  • Immunoglobulin Fab Fragments / ultrastructure
  • Microscopy, Electron*
  • Models, Structural*
  • Protein Conformation
  • Software*


  • Antigen-Antibody Complex
  • Calcium Channels
  • Immunoglobulin Fab Fragments
  • Hemocyanins