In vitro type-1 and type-2 cytokine production in systemic lupus erythematosus: lack of relationship with clinical disease activity

Lupus. 1996 Apr;5(2):139-45. doi: 10.1177/096120339600500209.

Abstract

Objective: To investigate the relationship between disease activity and in vitro cytokine, soluble(s)CD23 and polyclonal and anti-DNA antibody production by PBMC from patients with active systemic lupus erythematosus (SLE).

Methods: Cytokines, sCD23 and immunoglobulins were estimated by ELISA in unstimulated and polyclonal mitogen-stimulated culture supernatants.

Results: PHA-induced IL-2 and IFN-gamma production were decreased, whereas spontaneous and PHA-induced IL-6 and IL-10 production were increased in cultures of SLE lymphocytes. Conversely, spontaneous and PHA-stimulated IL-4 and sCD23 production was comparable between patients and controls. Finally, we found an increase in in vitro spontaneous polyclonal and anti-DNA IgG secretion.

Conclusions: We demonstrated an expanded type-2 cytokine profile with no correlation with parameters of disease activity.

Publication types

  • Comparative Study

MeSH terms

  • Adult
  • Antigens, CD / biosynthesis
  • Autoantibodies / biosynthesis*
  • CD3 Complex / biosynthesis
  • Cells, Cultured
  • Cytokines / biosynthesis*
  • DNA / immunology
  • Enzyme-Linked Immunosorbent Assay
  • Female
  • Humans
  • Immunoglobulin G / biosynthesis
  • Immunoglobulin M / biosynthesis
  • Interferon-gamma / biosynthesis
  • Interleukin-10 / biosynthesis
  • Interleukin-2 / biosynthesis
  • Interleukin-4 / biosynthesis
  • Interleukin-6 / biosynthesis
  • Lupus Erythematosus, Systemic / immunology*
  • Lupus Erythematosus, Systemic / physiopathology*
  • Lymphocyte Activation*
  • Lymphocytes / drug effects
  • Lymphocytes / immunology*
  • Male
  • Middle Aged
  • Receptors, IgE / biosynthesis
  • Reference Values

Substances

  • Antigens, CD
  • Autoantibodies
  • CD3 Complex
  • Cytokines
  • Immunoglobulin G
  • Immunoglobulin M
  • Interleukin-2
  • Interleukin-6
  • Receptors, IgE
  • Interleukin-10
  • Interleukin-4
  • Interferon-gamma
  • DNA