Uptake of acetazolamide by human erythrocytes in vitro

J Pharm Sci. 1977 May;66(5):729-31. doi: 10.1002/jps.2600660532.


The binding of acetazolamide to human erythrocytes was studied in vitro. Blood and plasma samples were analyzed by electron-capture GLC. At 37 degrees, drug equilibrated between plasma and erythrocytes in approximately 40 min. The effect of plasma concentration on the steady-state level of drug within the erythrocytes was examined. Erythrocyte accumulation of acetazolamide appeared to be a composite of two processes: a nonlinear, saturable process and a linear, diffusion-controlled process. By appropriate linear transformation of the data, estimates of the data, estimates of the erythrocyte binding capacity and the dissociation constant for the drug were obtained.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Acetazolamide / blood*
  • Binding Sites
  • Blood Proteins / metabolism
  • Chromatography, Gas
  • Erythrocytes / metabolism*
  • Humans
  • In Vitro Techniques
  • Protein Binding
  • Time Factors


  • Blood Proteins
  • Acetazolamide