The mae1 gene of Schizosaccharomyces pombe encodes a permease for malate and other C4 dicarboxylic acids

Yeast. 1995 Dec;11(15):1485-91. doi: 10.1002/yea.320111503.


The mae1 gene of the yeast Schizosaccharomyces pombe was identified on the basis of its ability to complement a mutant defective in the transport of malic acid. Analysis of the DNA sequence revealed an open reading frame of 1314 base pairs, encoding a polypeptide of 438 amino acids with a predicted molecular weight of 49 kDa. A hydropathy profile of the predicted amino acid sequence revealed a protein with ten membrane-spanning or associated domains and hydrophilic N- and C- termini. The predicted secondary structure of the protein in similar to models proposed for other integral membrane proteins from both prokaryotes and eukaryotes. The S. pombe mae1 gene encodes a single mRNA of 1.5 kb. The mea1 gene is expressed constitutively and is not subject to catabolite repression as was previously reported for the malate permease systems of Candida utilis and Hansenula anomala. The mae1 gene was mapped 2842 bp 5' to the MFml gene on chromosome I. Transport assays revealed that the mae1 gene encodes a permease involved in the uptake of L-malate, succinate and malonic acid.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • Biological Transport, Active
  • Chromosomes, Fungal / genetics
  • Cloning, Molecular
  • DNA, Fungal / genetics
  • Dicarboxylic Acids / metabolism*
  • Fermentation
  • Genes, Fungal*
  • Malates / metabolism*
  • Membrane Transport Proteins / chemistry
  • Membrane Transport Proteins / genetics*
  • Membrane Transport Proteins / metabolism*
  • Molecular Sequence Data
  • Open Reading Frames
  • Organic Anion Transporters / chemistry
  • Organic Anion Transporters / genetics*
  • Organic Anion Transporters / metabolism*
  • Protein Structure, Secondary
  • Restriction Mapping
  • Schizosaccharomyces / genetics*
  • Schizosaccharomyces / metabolism*
  • Schizosaccharomyces pombe Proteins / chemistry
  • Schizosaccharomyces pombe Proteins / genetics*
  • Schizosaccharomyces pombe Proteins / metabolism*


  • DNA, Fungal
  • Dicarboxylic Acids
  • MAE1 protein, S pombe
  • Malates
  • Membrane Transport Proteins
  • Organic Anion Transporters
  • Schizosaccharomyces pombe Proteins
  • malic acid

Associated data

  • GENBANK/U21002