Expression and localization of inhibin/activin subunits and activin receptors in MCF-7 cells, a human breast cancer cell line

Breast Cancer Res Treat. 1996;37(2):151-60. doi: 10.1007/BF01806496.

Abstract

Inhibins and activins are members of the transforming growth factor-beta (TGF-beta) superfamily. Since TGF beta has been shown to be a potent proliferation-inhibiting agent for the breast cancer cell line MCF-7, we determined whether this cell line (a) transcribes messenger RNAs coding inhibin/activin alpha-, beta A-, and beta B-subunits and activin receptors, and (b) produces inhibin and/or activin proteins. Messenger RNAs for alpha- and beta-subunits of inhibin/activin and activin receptor II in MCF-7 cells were detected and localized using the reverse transcription-polymerase chain reaction (RT-PCR) analysis and in situ hybridization, respectively. The identity of the RT-PCR products was confirmed by DNA sequencing of PCR products. Immunocytochemically, inhibin and activin were localized in these cells. Our findings that messenger RNAs encoding inhibin alpha-subunit, inhibin/activin beta A-subunit, and activin receptor II were expressed, and inhibin/activin proteins were produced by MCF-7 cells, imply that these gonadal growth factors may have paracrine/autocrine functions in human breast cancer. Further, these observations suggest that these growth factors may be involved in regulating the growth and differentiation of human breast cancer cells.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Activin Receptors
  • Activins
  • Adenocarcinoma / metabolism*
  • Base Sequence
  • Breast Neoplasms / metabolism*
  • Female
  • Humans
  • Immunohistochemistry
  • In Situ Hybridization
  • Inhibins / isolation & purification
  • Inhibins / metabolism*
  • Molecular Sequence Data
  • Polymerase Chain Reaction
  • RNA, Messenger / isolation & purification
  • RNA, Messenger / metabolism*
  • Receptors, Growth Factor / isolation & purification
  • Receptors, Growth Factor / metabolism*
  • Tumor Cells, Cultured

Substances

  • RNA, Messenger
  • Receptors, Growth Factor
  • Activins
  • Inhibins
  • Activin Receptors