Glia-dependent neurotoxicity and neuroprotection in mesencephalic cultures

Brain Res. 1995 Dec 15;704(1):112-6. doi: 10.1016/0006-8993(95)01189-7.

Abstract

Dopaminergic neurotoxicities of 6-hydroxydopamine (6-OHDA) and the lipopolysaccharide (LPS) were compared in rat mesencephalic cultures plated on poly-L-lysine or on glial monolayers. In the neuron-enriched cultures plated on polylysine, 6-OHDA killed 89% of the tyrosine hydroxylase (TH)-immunopositive neurons, but LPS was not neurotoxic. Conversely, in mixed neuron/glial cultures, 6-OHDA killed only 27% of the TH-immunopositive neurons while LPS killed 70%. The mixed neuronal/glial mesencephalic culture offers a better in vitro model for studying possible mechanisms involved in Parkinson's disease.

MeSH terms

  • Animals
  • Cell Count
  • Cells, Cultured
  • Lipopolysaccharides / toxicity*
  • Mesencephalon / cytology
  • Mesencephalon / drug effects*
  • Neuroglia / drug effects*
  • Neurons / drug effects
  • Neurons / enzymology
  • Neuroprotective Agents / metabolism*
  • Neurotoxins / toxicity*
  • Oxidopamine / toxicity*
  • Polylysine
  • Rats
  • Tyrosine 3-Monooxygenase / analysis

Substances

  • Lipopolysaccharides
  • Neuroprotective Agents
  • Neurotoxins
  • Polylysine
  • Oxidopamine
  • Tyrosine 3-Monooxygenase