The alpha(1,3)fucosyltransferase Fuc-TVII controls leukocyte trafficking through an essential role in L-, E-, and P-selectin ligand biosynthesis

Cell. 1996 Aug 23;86(4):643-53. doi: 10.1016/s0092-8674(00)80137-3.


alpha(1,3)Fucosylated oligosaccharides represent components of leukocyte counterreceptors for E- and P-selectins and of L-selectin ligands expressed by lymph node high endothelial venules (HEV). The identity of the alpha(1,3)fucosyltransferase(s) required for their expression has been uncertain, as has a requirement for alpha(1,3)fucosylation in HEV L-selectin ligand activity. We demonstrate here that mice deficient in alpha(1,3) fucosyltransferase Fuc-TVII exhibit a leukocyte adhesion deficiency characterized by absent leukocyte E- and P-selectin ligand activity and deficient HEV L-selectin ligand activity. Selectin ligand deficiency is distinguished by blood leukocytosis, impaired leukocyte extravasation in inflammation, and faulty lymphocyte homing. These observations demonstrate an essential role for Fuc-TVII in E-, P-, and L-selectin ligand biosynthesis and imply that this locus can control leukocyte trafficking in health and disease.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Antigens, Surface / metabolism
  • Cell Adhesion
  • Cell Movement
  • Cells, Cultured
  • E-Selectin / biosynthesis*
  • Endothelium, Vascular / physiology*
  • Fucosyltransferases / genetics*
  • Glycosylation
  • Immunologic Techniques
  • L-Selectin / biosynthesis*
  • Leukocytosis / genetics
  • Ligands
  • Lymphocytes / physiology*
  • Mice
  • Mice, Knockout
  • Neutrophils / physiology
  • P-Selectin / biosynthesis*
  • Protein Processing, Post-Translational


  • Antigens, Surface
  • E-Selectin
  • Ligands
  • P-Selectin
  • L-Selectin
  • Fucosyltransferases
  • galactoside 3-fucosyltransferase