Abstract
The AFG3 gene of Saccharomyces cerevisiae encodes a mitochondrial inner membrane protein with ATP-dependent protease activity. To gain more insight into the function of this protein, multi-copy suppressors of an afg3-null mutation were isolated. Three genes were found that restored partial growth on non-fermentable carbon sources, all of which affect the biogenesis of respiratory competent mitochondria: PIM1(LON) encodes a matrix-localized ATP-dependent protease involved in the turnover of matrix proteins; OXA1(PET1402) encodes a putative mitochondrial inner membrane protein involved in the biogenesis of the respiratory chain; and MBA1 encodes a mitochondrial protein required for optimal respiratory growth. All three genes also suppressed a null mutation in a related gene, RCA1, as well as in the combination of afg3- and rca1-null.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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ATP-Dependent Proteases
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Adenosine Triphosphatases / biosynthesis
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Adenosine Triphosphatases / genetics*
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Cell Nucleus / metabolism
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DNA, Mitochondrial / genetics
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Electron Transport Complex IV
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Fungal Proteins / biosynthesis
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Fungal Proteins / genetics*
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Gene Expression
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Genes, Fungal*
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Genomic Library
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Genotype
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Membrane Proteins / biosynthesis
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Membrane Proteins / genetics*
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Metalloendopeptidases*
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Mitochondria / genetics
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Mitochondrial Proteins
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Nuclear Proteins / biosynthesis
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Nuclear Proteins / genetics
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Restriction Mapping
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Saccharomyces cerevisiae / genetics*
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Saccharomyces cerevisiae / metabolism
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Saccharomyces cerevisiae Proteins*
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Serine Endopeptidases / biosynthesis
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Serine Endopeptidases / genetics
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Suppression, Genetic*
Substances
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DNA, Mitochondrial
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Fungal Proteins
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Membrane Proteins
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Mitochondrial Proteins
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Nuclear Proteins
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OXA1 protein
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Saccharomyces cerevisiae Proteins
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Electron Transport Complex IV
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ATP-Dependent Proteases
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PIM1 protein, S cerevisiae
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Serine Endopeptidases
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Metalloendopeptidases
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Adenosine Triphosphatases
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YTA12 protein, S cerevisiae
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AFG3 protein, S cerevisiae