Heteromultimerization and NMDA receptor-clustering activity of Chapsyn-110, a member of the PSD-95 family of proteins

Neuron. 1996 Jul;17(1):103-13. doi: 10.1016/s0896-6273(00)80284-6.

Abstract

Chapsyn-110, a novel membrane-associated putative guanylate kinase (MAGUK) that binds directly to N-methyl-D-aspartate (NMDA) receptor and Shaker K+ channel subunits, is 70%-80% identical to, and shares an identical domain organization with, PSD-95/SAP90 and SAP97. In rat brain, chapsyn-110 protein shows a somatodendritic expression pattern that overlaps partly with PSD-95 but that contrasts with the axonal distribution of SAP97. Chapsyn-110 associates tightly with the postsynaptic density in brain, and mediates the clustering of both NMDA receptors and K+ channels in heterologous cells. Indeed, chapsyn-110 and PSD-95 can heteromultimerize with each other and are recruited into the same NMDA receptor and K+ channel clusters. Thus, chapsyn-110 and PSD-95 may interact at postsynaptic sites to form a multimeric scaffold for the clustering of receptors, ion channels, and associated signalling proteins.

MeSH terms

  • Adaptor Proteins, Signal Transducing
  • Amino Acid Sequence
  • Animals
  • Brain / metabolism
  • Disks Large Homolog 4 Protein
  • Guanylate Kinases
  • Immunohistochemistry
  • Intracellular Signaling Peptides and Proteins
  • Membrane Proteins
  • Molecular Sequence Data
  • Nerve Tissue Proteins / chemistry
  • Nerve Tissue Proteins / physiology*
  • Nucleoside-Phosphate Kinase / chemistry
  • Nucleoside-Phosphate Kinase / genetics
  • Nucleoside-Phosphate Kinase / physiology*
  • Potassium Channels / physiology
  • Rabbits
  • Rats
  • Receptor Aggregation*
  • Receptors, N-Methyl-D-Aspartate / physiology*
  • Subcellular Fractions / metabolism
  • Tissue Distribution
  • Tumor Suppressor Proteins

Substances

  • Adaptor Proteins, Signal Transducing
  • DLG1 protein, human
  • Disks Large Homolog 4 Protein
  • Dlg1 protein, rat
  • Dlg4 protein, rat
  • Intracellular Signaling Peptides and Proteins
  • Membrane Proteins
  • Mpp2 protein, rat
  • Nerve Tissue Proteins
  • Potassium Channels
  • Receptors, N-Methyl-D-Aspartate
  • Tumor Suppressor Proteins
  • postsynaptic density proteins
  • Nucleoside-Phosphate Kinase
  • DLG2 protein, human
  • Guanylate Kinases

Associated data

  • GENBANK/U32376
  • GENBANK/U49049