Tissue-specific expression of the PAL3 promoter requires the interaction of two conserved cis sequences

Plant Mol Biol. 1996 May;31(2):393-7. doi: 10.1007/BF00021800.

Abstract

The bean PAL2 and PAL3 promoters confer expression in overlapping sets of tissue types in transgenic tobacco. The PAL3 promoter contains motifs that resemble two AC cis elements which are required for tissue-specific expression of the PAL2 promoter. The functions of these motifs in the PAL3 promoter were determined by analysis of mutated PAL3 promoter-GUS constructs in transgenic tobacco. This revealed that the AC motifs are necessary for tissue-specific expression of the PAL3 promoter. Therefore, a key role is indicated for AC elements, which are Myb-protein binding sites, in regulating tissue-specific expression of the bean PAL gene family.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Base Sequence
  • Consensus Sequence
  • DNA Mutational Analysis
  • Fabaceae / genetics*
  • Gene Expression Regulation, Enzymologic
  • Gene Expression Regulation, Plant*
  • Molecular Sequence Data
  • Phenylalanine Ammonia-Lyase / genetics
  • Plants, Genetically Modified
  • Plants, Medicinal*
  • Plants, Toxic
  • Promoter Regions, Genetic*
  • Tissue Distribution
  • Tobacco

Substances

  • Phenylalanine Ammonia-Lyase