A rapid screening procedure to identify mini-Tn10 insertion mutants of Escherichia coli K-12 with altered adhesion properties

FEMS Microbiol Lett. 1996 Aug 15;142(1):27-30. doi: 10.1111/j.1574-6968.1996.tb08402.x.

Abstract

A rapid screening procedure was developed for detection of Escherichia coli mutants with altered adhesion abilities using polystyrene 96-well microtiter plates as attachment surfaces. During this assay, bacterial strains grew and adhered simultaneously, and attached cells were measured after crystal violet staining. Starting with a total of 7000 W3110::Tn10 insertion mutants of E. coli K-12 W3110, 50 adhesion-deficient mutants were isolated which showed less than 40% attachment, and 22 mutants were found with an attachment of 40-75%. Motility assays were performed on these 72 mutants, and 34 displayed altered motility.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacterial Adhesion / genetics*
  • Bacteriological Techniques
  • Biofilms
  • DNA Transposable Elements*
  • Escherichia coli / genetics*
  • Evaluation Studies as Topic
  • Mutation*

Substances

  • DNA Transposable Elements