Denaturing gradient gel electrophoresis: a novel method for determining Rh phenotype from genomic DNA

Br J Haematol. 1996 Aug;94(2):417-21. doi: 10.1046/j.1365-2141.1996.d01-1808.x.


Denaturing gradient gel electrophoresis (DGGE) was carried out on PCR products amplified from exons 2 and 5 of RHD and RHCE. Exon 2 of RHD and exon 2 of the C allele of RHCE have an identical sequence, which differs from that of the c allele of RHCE. One band representing D and/or C, and another representing c, could be distinguished by DGGE of exon 2 amplifications of genomic DNA from individuals with the appropriate Rh phenotype. C and c could only be distinguished in D-negative samples. Exon 5 of RHD and exon 5 of the E and e alleles of RHCE all have different nucleotide sequences. Bands representing D, E and e could be distinguished following DGGE of the products of exon 5 amplification of genomic DNA from individuals with red cells of the appropriate Rh phenotype. In samples from individuals with VS+ red cells (V+ or V-) there was a shift of the band representing e. Sequencing demonstrated that VS is associated with a RHCE e sequence with a single base change predicting a Leu245 --> Val substitution in the Rh polypeptide. This substitution may be responsible for the VS and e5 antigens.

MeSH terms

  • Base Sequence
  • DNA / genetics*
  • DNA Mutational Analysis
  • Electrophoresis / methods*
  • Exons
  • Humans
  • Molecular Sequence Data
  • Mutation
  • Nucleic Acid Heteroduplexes
  • Phenotype
  • Polymerase Chain Reaction
  • Rh-Hr Blood-Group System / genetics*
  • Rh-Hr Blood-Group System / immunology


  • Nucleic Acid Heteroduplexes
  • Rh-Hr Blood-Group System
  • DNA