Reciprocal GLUT-1 and GLUT-4 expression and glucose transport in denervated muscles

Am J Physiol. 1996 Jul;271(1 Pt 1):E50-7. doi: 10.1152/ajpendo.1996.271.1.E50.

Abstract

We investigated in 3-day-denervated muscles 1) the expression of GLUT-1 in perineurial sheaths (PNS) and muscle, 2) the muscle fiber-specific changes in GLUT-1 and GLUT-4, and 3) changes in basal and insulin-stimulated 3-O-methylglucose transport. GLUT-1 was increased in both the PNS (P < 0.05) and in the muscle membranes (P < 0.05). GLUT-1 and GLUT-4 concentrations were changed reciprocally, in a fiber-dependent fashion [GLUT-1: red gastrocnemius (RG), +31%; white gastrocnemius (WG), +10%; GLUT-4: RG, -53%; WG, -16%]. Basal glucose transport was increased (P < 0.05), and this increase was correlated with the oxidative nature of the muscles (r = 0.97). Insulin-stimulated glucose transport was decreased in denervated muscles (P < 0.05). This was also related to the oxidative nature of the muscles (r = -0.88). The increase in basal glucose transport was correlated with the loss of insulin-stimulated transport (r = 0.95). Thus the increase in GLUT-1 compensates for the loss of GLUT-4, resulting in a 56% regain of the reduced insulin-stimulated glucose transport.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 3-O-Methylglucose / metabolism
  • Animals
  • Biological Transport / drug effects
  • Glucose / metabolism*
  • Glucose Transporter Type 1
  • Glucose Transporter Type 4
  • Insulin / pharmacology
  • Male
  • Monosaccharide Transport Proteins / metabolism*
  • Muscle Denervation*
  • Muscle Proteins*
  • Muscles / metabolism*
  • Oxidation-Reduction
  • Rats
  • Rats, Sprague-Dawley

Substances

  • Glucose Transporter Type 1
  • Glucose Transporter Type 4
  • Insulin
  • Monosaccharide Transport Proteins
  • Muscle Proteins
  • Slc2a1 protein, rat
  • Slc2a4 protein, rat
  • 3-O-Methylglucose
  • Glucose