Hindbrain GRP receptor blockade antagonizes feeding suppression by peripherally administered GRP

Am J Physiol. 1996 Jul;271(1 Pt 2):R180-4. doi: 10.1152/ajpregu.1996.271.1.R180.


Bombesin (BN)-like peptides injected peripherally or centrally suppress food intake in rats. The relationship between the central and peripheral actions of BN is unknown. However, experimental evidence supports a critical role for the caudal hindbrain in mediating the feeding effects of BN. To investigate this relationship further, we examined the ability of fourth ventricular infusion of a specific gastrin-releasing peptide (GRP) antagonist, [D-F5, Phe6, D-Ala11]BN-(6-13) methyl ester (BN-ME), to block suppression of glucose intake (0.5 kcal/ml) produced by intraperitoneal administration of GRP-(18-27) in 5-h food-deprived male Sprague-Dawley rats (n = 10). We found that fourth ventricular administration of 10, 32, and 100 ng BN-ME blocked the suppression of glucose intake produced by peripherally administered 10 nmol/kg GRP-(18-27). The most effective dose of BN-ME (100 ng) blocked the ability of peripheral injection of GRP-(18-27) to inhibit glucose intake but had no effect on intake when given alone. These results demonstrate that the availability of caudal hindbrain GRP receptors is necessary for peripherally administered GRP-(18-27) to reduce food intake in rats.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Bombesin / analogs & derivatives
  • Bombesin / pharmacology
  • Eating / drug effects*
  • Food Deprivation
  • Gastrin-Releasing Peptide
  • Gastrins / pharmacology
  • Injections, Intraperitoneal
  • Injections, Intravenous
  • Injections, Intraventricular
  • Male
  • Peptide Fragments / pharmacology
  • Peptides / antagonists & inhibitors
  • Peptides / pharmacology*
  • Rats
  • Rats, Sprague-Dawley
  • Receptors, Bombesin / antagonists & inhibitors*
  • Rhombencephalon / metabolism*
  • Satiety Response / physiology


  • BIM 26226
  • Gastrins
  • Peptide Fragments
  • Peptides
  • Receptors, Bombesin
  • Gastrin-Releasing Peptide
  • neuromedin C
  • Bombesin