Endogenous reverse transcription assays reveal high-level resistance to the triphosphate of (-)2'-dideoxy-3'-thiacytidine by mutated M184V human immunodeficiency virus type 1

J Virol. 1996 Aug;70(8):5642-5. doi: 10.1128/JVI.70.8.5642-5645.1996.


Kinetic analysis showed that the Ki values and the Ki/Km ratios for mutated, recombinant M184V human immunodeficiency virus type 1 reverse transcriptase (RT) for (-)2'-dideoxy-3'-thiacytidine triphosphate (3TCTP) were 35-fold higher than the equivalent values for wild-type RT but only about twice as high as the equivalent values for each of the triphosphates of ddC (ddCTP) and ddA (ddATP). Fully endogenous RT assays showed that viruses containing the M184V substitution were highly resistant to 3TCTP, with an increase in the 50% inhibitory concentration of 250-fold in comparison with wild-type recombinant virus.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Anti-HIV Agents / pharmacology*
  • Deoxyadenine Nucleotides / metabolism
  • Deoxyadenine Nucleotides / pharmacology*
  • Dideoxynucleotides
  • Drug Resistance
  • HIV Reverse Transcriptase / genetics*
  • HIV Reverse Transcriptase / metabolism
  • HIV-1 / enzymology*
  • HIV-1 / genetics
  • Humans
  • Lamivudine / metabolism
  • Lamivudine / pharmacology*
  • Mutation
  • Recombinant Proteins / genetics
  • Recombinant Proteins / metabolism
  • Transcription, Genetic / drug effects


  • Anti-HIV Agents
  • Deoxyadenine Nucleotides
  • Dideoxynucleotides
  • Recombinant Proteins
  • 2',3'-dideoxyadenosine triphosphate
  • Lamivudine
  • HIV Reverse Transcriptase