Skip to main page content
Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
, 56 (14), 3168-72

BRCA1 Is a 220-kDa Nuclear Phosphoprotein That Is Expressed and Phosphorylated in a Cell Cycle-Dependent Manner

Affiliations
  • PMID: 8764100

BRCA1 Is a 220-kDa Nuclear Phosphoprotein That Is Expressed and Phosphorylated in a Cell Cycle-Dependent Manner

Y Chen et al. Cancer Res.

Erratum in

  • Cancer Res 1996 Sep 1;56(17):4074

Abstract

Mouse polyclonal antibodies, raised against three regions of the human BRCA1 protein, were characterized and revealed BRCA1 as a 220-kDa nuclear phosphoprotein in normal cells. All three antisera recognize both in vitro-translated and recombinant, baculovirus-derived BRCA1, which co-migrate with BRCA1 from the human breast epithelia cell line, HBL100. BRCA1 expression and phosphorylation are shown to be cell cycle dependent, with greatest expression and phosphorylation occurring in S and M phases. Cyclin-dependent kinase 2 and other kinases associated with cyclins D and A are shown to bind to and phosphorylate BRCA1, suggesting that the biological activity of BRCA1 may be regulated by cyclin-dependent kinases.

Similar articles

See all similar articles

Cited by 71 PubMed Central articles

See all "Cited by" articles

Publication types

LinkOut - more resources

Feedback