The multiple-sugar metabolism (msm) gene cluster of Streptococcus mutans is transcribed as a single operon

FEMS Microbiol Lett. 1996 Jul 1;140(2-3):261-4. doi: 10.1016/0378-1097(96)00191-7.

Abstract

The multiple-sugar metabolism (msm) locus of Streptococcus mutans constitutes a non-PTS sugar uptake system responsible for the transport and utilization of raffinose, melibiose and isomaltotrioses. While previous studies have used polar mutations to suggest that these genes are co-transcribed, there has not been evidence to support this. In this report we present direct evidence that the msm genes can be transcribed as a single operon.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Base Sequence
  • Biological Transport, Active / genetics
  • Carbohydrate Metabolism*
  • Carbohydrates / genetics*
  • DNA, Bacterial / genetics
  • DNA, Complementary / genetics
  • Genes, Bacterial*
  • Melibiose / metabolism
  • Molecular Sequence Data
  • Multigene Family*
  • Operon*
  • Polymerase Chain Reaction
  • RNA, Bacterial / genetics
  • RNA, Bacterial / isolation & purification
  • Raffinose / metabolism
  • Streptococcus mutans / genetics*
  • Streptococcus mutans / metabolism*
  • Transcription, Genetic
  • Trisaccharides / metabolism

Substances

  • Carbohydrates
  • DNA, Bacterial
  • DNA, Complementary
  • RNA, Bacterial
  • Trisaccharides
  • isomaltotriose
  • Melibiose
  • Raffinose