Redirecting the complete T cell receptor/CD3 signaling machinery towards native antigen via modified T cell receptor

Eur J Immunol. 1996 Aug;26(8):1770-4. doi: 10.1002/eji.1830260816.

Abstract

We show that a chimeric T cell receptor (TCR) beta chain consisting of a single-chain Fv portion derived from a monoclonal antibody and the full TCR beta chain is able to assemble functionally with endogenous TCR/CD3 components and transfer the antibody specificity as well as the TCR specificity into TCR beta- as well as into TCR beta+ T cells. This allows the incorporation new non-major histocompatibility complex-restricted ligand specificities into the intact TCR/CD3 complex which can exploit the full range of biological activities of the endogenous TCR signaling machinery. This approach can provide wider opportunities to redirect T cells to virus or tumor antigen-bearing cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Hemagglutinins, Viral / chemistry
  • Hemagglutinins, Viral / immunology
  • Hemagglutinins, Viral / pharmacology
  • Histocompatibility Antigens Class II / pharmacology
  • Humans
  • Hybridomas
  • Immunoglobulin Fragments / chemistry
  • Influenza A virus / chemistry
  • Influenza A virus / immunology
  • Leukemia, T-Cell
  • Mice
  • Mice, Inbred AKR
  • Models, Molecular
  • Molecular Sequence Data
  • Peptides / pharmacology
  • Receptor-CD3 Complex, Antigen, T-Cell / chemistry*
  • Receptor-CD3 Complex, Antigen, T-Cell / genetics
  • Receptor-CD3 Complex, Antigen, T-Cell / immunology*
  • Receptors, Antigen, T-Cell, alpha-beta / chemistry*
  • Receptors, Antigen, T-Cell, alpha-beta / genetics
  • Receptors, Antigen, T-Cell, alpha-beta / immunology*
  • Signal Transduction / immunology*
  • Transfection / immunology
  • Tumor Cells, Cultured

Substances

  • Hemagglutinins, Viral
  • Histocompatibility Antigens Class II
  • I-E-antigen
  • Immunoglobulin Fragments
  • Peptides
  • Receptor-CD3 Complex, Antigen, T-Cell
  • Receptors, Antigen, T-Cell, alpha-beta
  • immunoglobulin Fv