CD32 expression and signaling is down-regulated by transforming growth factor-beta 1 on human monocytes

Eur J Immunol. 1996 Aug;26(8):1970-3. doi: 10.1002/eji.1830260844.


CD32 (Fc gamma RII) is the most abundantly distributed class of IgG Fc receptors in the human body. In this study, we analyzed the effect of transforming growth factor (TGF)-beta 1, a cytokine with strong immunosuppressive function, on the expression and function of CD32 on freshly isolated peripheral blood monocytes and three human monocytic cell lines, U937, THP-1 and Mono mac-6. We found that TGF-beta 1 down-regulates CD32 expression on monocytes and all monocytic cell lines in a dose- and time-dependent fashion. A mean down-regulation of CD32 expression on THP-1 cells of 54 +/- 3.2% after 24 h was found at a concentration of 1 ng/ml TGF-beta 1. At the mRNA level, TGF-beta 1 induced a twofold down-regulation of CD32. Cross-linking of CD32 induced an increase in the concentration of intracellular Ca2+, which was reduced by 50% by TGF-beta 1, suggesting a decreased downstream signaling mediated by the receptor.

MeSH terms

  • Antigens, Surface / biosynthesis
  • Cross-Linking Reagents
  • Down-Regulation / drug effects
  • Down-Regulation / immunology*
  • Humans
  • Monocytes / drug effects*
  • Monocytes / immunology
  • Monocytes / metabolism*
  • RNA, Messenger / biosynthesis
  • Receptors, IgG / biosynthesis*
  • Receptors, IgG / metabolism
  • Signal Transduction / drug effects
  • Signal Transduction / immunology*
  • Transforming Growth Factor beta / pharmacology*


  • Antigens, Surface
  • Cross-Linking Reagents
  • RNA, Messenger
  • Receptors, IgG
  • Transforming Growth Factor beta