Lysozymes, related to the chicken-type lysozymes in vertebrates, are ubiquitous components in the bacteriolytic armamentarium of insects. The enzyme is normally present in the blood, and together with other bactericidal factors lysozyme is often strongly induced when the insect is infected. This response is regulated by mechanisms that are related to those that activate inflammatory, acute-phase and immune responses in mammals, and the induction of lysozyme and other factors is now being investigated as a model for innate immune reactions in general. A special adaptation is seen in flies like Musca and Drosophila. These animals live on the microorganisms in decompositing matter, and they have developed a specialized set of lysozymes that are expressed in the alimentary tract. In Drosophila, at least seven different lysozyme genes are clustered in a small region on the third chromosome. The different genes are expressed in different parts of the digestive tract, and at different time points during development, and they are highly divergent in sequence. The major lysozymes in the fly gut have acidic isoelectric points and/or pH optima, and their evolution provides an interesting parallel to the ruminants.