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Comparative Study
. 1996 Jun 21;287(2):139-55.
doi: 10.1016/0008-6215(96)00067-5.

Analysis of Charged Cyclomalto-Oligosaccharides (Cyclodextrin) Derivatives by Ion-Spray, Matrix-Assisted Laser-Desorption/Ionization Time-Of-Flight and Fast-Atom Bombardment Mass Spectrometry, and by Capillary Electrophoresis

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Comparative Study

Analysis of Charged Cyclomalto-Oligosaccharides (Cyclodextrin) Derivatives by Ion-Spray, Matrix-Assisted Laser-Desorption/Ionization Time-Of-Flight and Fast-Atom Bombardment Mass Spectrometry, and by Capillary Electrophoresis

B Chankvetadze et al. Carbohydr Res. .

Abstract

The use of electrospray-ionization mass spectrometry (ESIMS), matrix-assisted laser-desorption/ionization time-of-flight mass spectrometry (MALDITOFMS), and fast-atom bombardment mass spectrometry (FABMS) for the rapid determination of molecular weight, degree of substitution (ds), and purity is demonstrated for charged derivatives of cyclomalto-heptaose (beta CD) and -octaose (gamma CD). The access to anionic sulfoalkyl ethers (alkyl: ethyl, n-propyl, and n-butyl) and to beta CD-2-hydroxy-3-trimethylammoniumpropyl ether chlorides (HTAP-beta CD) leads only to mixtures of products, the compositions of which can be determined directly from ESI and MALDITOF mass spectra. All charged derivatives consist of a mixture of unreacted and higher substituted compounds. The substitution patterns obtained by MS are in good agreement with the results of experiments on the separation of beta CD-sulfoalkyl ethers by capillary electrophoresis (CE).

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