Suppressing effects of 6-(2,5-dichlorophenyl)-2,4-diamino-1,3,5-triazine and related synthetic compounds on azoxymethane-induced aberrant crypt foci in rat colon

Jpn J Cancer Res. 1996 Jun;87(6):549-54. doi: 10.1111/j.1349-7006.1996.tb00258.x.

Abstract

The modifying effects of dietary administration of 6-(2,5-dichlorophenyl)-2,4-diamino-1,3,5-triazine and 5 related compounds on the occurrence of azoxymethane (AOM)-induced colonic aberrant crypt foci (ACF) were investigated in rats. Male F344 rats were given s.c. injections of AOM (15 mg/kg body weight) once a week for 3 weeks to induce ACF. They also received the diet containing 200 ppm test compound for 5 weeks, starting one week before the first dosing of AOM. At the termination of experiment, all of the compounds had caused a significant reduction in ACF frequency, which might be associated with suppression of the expression of proliferation biomarkers. The apoptotic index in the colonic mucosal epithelium of rats killed at 6 h after the first AOM exposure revealed no blocking activity of the compounds.

MeSH terms

  • Animals
  • Anticarcinogenic Agents / administration & dosage
  • Anticarcinogenic Agents / therapeutic use*
  • Apoptosis
  • Azoxymethane*
  • Colonic Neoplasms / chemically induced*
  • Colonic Neoplasms / pathology
  • Colonic Neoplasms / prevention & control*
  • Diet
  • Epithelium / pathology
  • Intestinal Mucosa / pathology
  • Male
  • Ornithine Decarboxylase / metabolism
  • Polyamines / blood
  • Precancerous Conditions / chemically induced*
  • Precancerous Conditions / pathology
  • Precancerous Conditions / prevention & control*
  • Rats
  • Rats, Inbred F344
  • Triazines / administration & dosage
  • Triazines / therapeutic use*

Substances

  • Anticarcinogenic Agents
  • Polyamines
  • Triazines
  • Ornithine Decarboxylase
  • Azoxymethane
  • irsogladine