Activated T cells induce expression of E-selectin in vitro and in an antigen-dependent manner in vivo

Eur J Immunol. 1996 Jul;26(7):1571-9. doi: 10.1002/eji.1830260725.


E-selectin is an endothelial adhesion molecule for polymorphonuclear cells, monocytes and skin-homing T cells. We have analyzed whether murine T cells are able to induce expression of E-selectin in vitro and in vivo. Using models of inflammation in which T cells play either a significant or only a minor role, we compared induction of E-selectin between normal mice and mice lacking functional T cells (athymic nude mice). In irritant contact dermatitis, a model without a major role for T cells, E-selectin was transiently expressed within the first 24 h in both normal and nude mice. In experimental leishmaniasis (where specific T cells play an important role), a high expression of E-selectin was maintained for 48 h in normal mice, whereas in nude mice expression was only transient. However, reconstitution of nude mice with 10(8) T cells from draining lymph nodes (LN) of Leishmania-infected normal mice could restore sustained expression of E-selectin. Transfer of T lymphocytes from normal LN or from LN of mice sensitized to the contact allergen trinitrochlorobenzene (TNCB) did not have this effect. T cells from TNCB-sensitized mice, however, did induce sustained expression of E-selectin in nude mice when TNCB was applied locally; here, reconstitution with Leishmania-specific T cells had no effect. In vitro, T cells from infected or TNCB-sensitized normal mice increased expression of E-selectin on microvascular endothelial cells after 4 h of co-culture. T cells from untreated mice were less effective. Induction was dependent on direct cell-cell contact, but not on the action of interleukin-1 alpha, interleukin-1 beta, tumor necrosis factor-alpha or interferon-gamma. We conclude that sensitized T cells induce sustained expression of E-selectin in vivo in an antigen-dependent manner. This novel way of regulation could be relevant for cell-mediated immunity and chronic disease. The mechanisms are unknown, but, as in vitro, might require direct cell-cell contact.

MeSH terms

  • Animals
  • Antigens / pharmacology
  • Cell Communication / immunology
  • Cells, Cultured
  • E-Selectin / biosynthesis*
  • E-Selectin / drug effects
  • E-Selectin / immunology
  • Endothelium, Vascular / cytology
  • Endothelium, Vascular / immunology
  • Interleukin-1 / pharmacology
  • Leishmaniasis, Cutaneous / immunology
  • Lymphocyte Activation*
  • Mice
  • Mice, Inbred BALB C
  • Mice, Nude
  • Picryl Chloride / immunology
  • T-Lymphocytes / immunology
  • T-Lymphocytes / metabolism*
  • Tumor Necrosis Factor-alpha / pharmacology


  • Antigens
  • E-Selectin
  • Interleukin-1
  • Tumor Necrosis Factor-alpha
  • Picryl Chloride