Abstract
The global regulators agr and sar control expression of cell wall and extracellular proteins. Inactivation of either sar and/or agr in a typical heterogeneously methicillin-resistant Staphylococcus aureus resulted in a small but reproducible decrease in the number of cells in the subpopulation expressing high methicillin resistance. The amount of low affinity penicillin-binding protein PBP2', the prerequisite for methicillin resistance, was apparently not affected, however, a reduction in PBP1 and PBP3 production was observed, suggesting that these resident PBPs of the cells might be involved somehow together with PBP2' in high level methicillin resistance.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Bacterial Proteins / genetics*
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Carrier Proteins / physiology
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Gene Expression Regulation, Bacterial
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Hexosyltransferases*
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Methicillin / pharmacology*
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Muramoylpentapeptide Carboxypeptidase / physiology
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Penicillin Resistance / genetics*
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Penicillin-Binding Proteins
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Penicillins / pharmacology*
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Peptidyl Transferases*
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Staphylococcus aureus / drug effects*
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Staphylococcus aureus / genetics*
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Trans-Activators*
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Transcription Factors / genetics*
Substances
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Agr protein, Staphylococcus aureus
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Bacterial Proteins
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Carrier Proteins
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Penicillin-Binding Proteins
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Penicillins
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SarA protein, bacterial
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Trans-Activators
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Transcription Factors
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Peptidyl Transferases
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Hexosyltransferases
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Muramoylpentapeptide Carboxypeptidase
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Methicillin