Cloning and expression of a human placenta inositol 1,3,4,5-tetrakisphosphate and phosphatidylinositol 3,4,5-trisphosphate 5-phosphatase

Biochem Biophys Res Commun. 1996 Aug 5;225(1):243-9. doi: 10.1006/bbrc.1996.1161.


Distinct inositol and phosphatidylinositol polyphosphate 5-phosphatases have recently been cloned. Primers were designated coding for highly conserved amino acid regions that are shared between sequences of 5-phosphatases. We used degenerate primers to amplify polymerase chain reaction products from rat brain cDNA. A product with a novel sequence was identified and used to clone a 4.9 kb cDNA from human placenta cDNA libraries (hp51CN). COS-7 cells transfected with a C-terminal truncated form of this cDNA showed an increase in Ins(1,3,4,5)P4 and PtdIns(3,4,5)P3 hydrolyzing activity, but not in Ins(1,4,5)P3 5-phosphatase. Enzymatic activity was inhibited in the presence of 2,3-bisphosphoglycerate and p-hydroxymercuribenzoate. The presence of an SH2 domain and proline-rich sequence motifs within hp51CN suggests that this 5-phosphatase interacts with various proteins in signal transduction.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Brain / enzymology*
  • Cell Line
  • Chlorocebus aethiops
  • Cloning, Molecular
  • Conserved Sequence
  • DNA Primers
  • Female
  • Gene Expression
  • Humans
  • Inositol Polyphosphate 5-Phosphatases
  • Kinetics
  • Molecular Sequence Data
  • Organ Specificity
  • Phosphoric Monoester Hydrolases / biosynthesis*
  • Phosphoric Monoester Hydrolases / metabolism
  • Placenta / enzymology*
  • Polymerase Chain Reaction
  • Pregnancy
  • Rats
  • Recombinant Proteins / biosynthesis
  • Recombinant Proteins / metabolism
  • Substrate Specificity
  • Transfection
  • src Homology Domains


  • DNA Primers
  • Recombinant Proteins
  • phosphatidylinositol-3,4,5-trisphosphate 5-phosphatase
  • Phosphoric Monoester Hydrolases
  • Inositol Polyphosphate 5-Phosphatases

Associated data

  • GENBANK/L36818
  • GENBANK/X98429