Abstract
Whereas the human killer cell inhibitory receptors (KIRs) for HLA class I are immunoglobulin-like monomeric type I glycoproteins, the murine Ly49 receptors for H-2 are type II homodimers of the C-type lectin superfamily. Here, we demonstrate that human NK cells also express C-type lectin receptors that influence recognition of polymorphic HLA-A, HLA-B, and HLA-C molecules. These receptors are heterodimers composed of CD94 chains covalently associated with novel tyrosine-phosphorylated glycoproteins (94AP). Some NK clones recognize a common HLA-C ligand using both KIRs and CD94-94AP receptors. These findings suggest the existence of human inhibitory MHC class I receptors of the immunoglobulin and C-type lectin superfamilies and indicate overlap in ligand specificity.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Adult
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Antibodies, Monoclonal / pharmacology
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Antigens, CD / immunology*
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Antigens, CD / metabolism*
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Clone Cells
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Glycoproteins / immunology
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Glycoproteins / metabolism
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HLA-A Antigens / immunology
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HLA-B Antigens / immunology
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HLA-C Antigens / immunology
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Histocompatibility Antigens Class I / immunology*
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Humans
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Isoantigens / immunology*
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Killer Cells, Natural / immunology
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Killer Cells, Natural / metabolism*
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Lectins / metabolism*
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Lectins, C-Type*
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Ligands
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Membrane Glycoproteins / immunology*
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Membrane Glycoproteins / metabolism*
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NK Cell Lectin-Like Receptor Subfamily D
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Phosphorylation
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Protein Binding / immunology
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Receptors, Immunologic / physiology*
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Tyrosine / metabolism
Substances
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Antibodies, Monoclonal
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Antigens, CD
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Glycoproteins
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HLA-A Antigens
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HLA-B Antigens
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HLA-C Antigens
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Histocompatibility Antigens Class I
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Isoantigens
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KLRD1 protein, human
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Lectins
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Lectins, C-Type
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Ligands
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Membrane Glycoproteins
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NK Cell Lectin-Like Receptor Subfamily D
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Receptors, Immunologic
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Tyrosine