CD94 and a novel associated protein (94AP) form a NK cell receptor involved in the recognition of HLA-A, HLA-B, and HLA-C allotypes

Immunity. 1996 Aug;5(2):163-72. doi: 10.1016/s1074-7613(00)80492-6.


Whereas the human killer cell inhibitory receptors (KIRs) for HLA class I are immunoglobulin-like monomeric type I glycoproteins, the murine Ly49 receptors for H-2 are type II homodimers of the C-type lectin superfamily. Here, we demonstrate that human NK cells also express C-type lectin receptors that influence recognition of polymorphic HLA-A, HLA-B, and HLA-C molecules. These receptors are heterodimers composed of CD94 chains covalently associated with novel tyrosine-phosphorylated glycoproteins (94AP). Some NK clones recognize a common HLA-C ligand using both KIRs and CD94-94AP receptors. These findings suggest the existence of human inhibitory MHC class I receptors of the immunoglobulin and C-type lectin superfamilies and indicate overlap in ligand specificity.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Adult
  • Antibodies, Monoclonal / pharmacology
  • Antigens, CD / immunology*
  • Antigens, CD / metabolism*
  • Clone Cells
  • Glycoproteins / immunology
  • Glycoproteins / metabolism
  • HLA-A Antigens / immunology
  • HLA-B Antigens / immunology
  • HLA-C Antigens / immunology
  • Histocompatibility Antigens Class I / immunology*
  • Humans
  • Isoantigens / immunology*
  • Killer Cells, Natural / immunology
  • Killer Cells, Natural / metabolism*
  • Lectins / metabolism*
  • Lectins, C-Type*
  • Ligands
  • Membrane Glycoproteins / immunology*
  • Membrane Glycoproteins / metabolism*
  • NK Cell Lectin-Like Receptor Subfamily D
  • Phosphorylation
  • Protein Binding / immunology
  • Receptors, Immunologic / physiology*
  • Tyrosine / metabolism


  • Antibodies, Monoclonal
  • Antigens, CD
  • Glycoproteins
  • HLA-A Antigens
  • HLA-B Antigens
  • HLA-C Antigens
  • Histocompatibility Antigens Class I
  • Isoantigens
  • KLRD1 protein, human
  • Lectins
  • Lectins, C-Type
  • Ligands
  • Membrane Glycoproteins
  • NK Cell Lectin-Like Receptor Subfamily D
  • Receptors, Immunologic
  • Tyrosine