Vitamin D receptor and growth inhibition by 1,25-dihydroxyvitamin D3 in human malignant melanoma cell lines

J Surg Res. 1996 Feb 15;61(1):127-33. doi: 10.1006/jsre.1996.0092.


The expression of vitamin D receptors (VDR) and growth inhibition induced by 1,25-dihydroxyvitamin D3 have been noted in certain human malignant melanoma cell lines. In this study, widely disparate levels of VDR mRNA expression were demonstrated in a panel of eight human malignant melanoma cell lines. Quantitation of receptor level by ligand binding assay showed a similar pattern. Proliferation and growth curve analysis was performed in two cell lines: RPMI 7951 (high VDR) and SK-MEL-28 (low VDR). Significant growth inhibition was noted in RPMI 7951 cells at 10(-9) M 1,25-dihydroxyvitamin D3. SK-MEL-28 cells, which express much lower levels of VDR, did not show any growth inhibition except at extremely high concentrations of 1,25-dihydroxyvitamin D3, namely 10(-5) M. These findings suggest a receptor-mediated mechanism of growth inhibition for 1,25-dihydroxyvitamin D3 and a role for this hormone in the growth of malignant melanoma cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Calcitriol / pharmacology*
  • Cell Division / drug effects
  • Dose-Response Relationship, Drug
  • Humans
  • Melanoma / metabolism*
  • Melanoma / pathology*
  • Receptors, Calcitriol / metabolism*
  • Tumor Cells, Cultured


  • Receptors, Calcitriol
  • Calcitriol