Generation of a murine monoclonal antibody specific for N-glycolylneuraminic acid-containing gangliosides that also recognizes sulfated glycolipids

Hybridoma. 1995 Dec;14(6):551-6. doi: 10.1089/hyb.1995.14.551.


The P3 murine monoclonal antibody (MAb) was generated by immunizing BALB/c mice with NeuGcGM3 included into liposomes. The specificity of this MAb was defined by an enzyme-linked immunosorbent assay and immunostaining on thin-layer chromatograms. P3 MAb binds to NeuGc-containing gangliosides and was shown also to react with sulfated glycolipids. A preliminary immunohistochemical study showed that the P3 MAb was able to recognize antigens expressed in human breast tumors.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antibodies, Monoclonal / biosynthesis*
  • Antibodies, Monoclonal / chemistry
  • Antibody Specificity*
  • Carbohydrate Sequence
  • Gangliosides / immunology*
  • Glycolipids / immunology*
  • Glycolipids / metabolism
  • Humans
  • Immunohistochemistry
  • Mice
  • Mice, Inbred BALB C
  • Molecular Sequence Data
  • Neuraminic Acids / analysis*
  • Neuraminic Acids / immunology
  • Organ Specificity / immunology
  • Sulfoglycosphingolipids / immunology


  • Antibodies, Monoclonal
  • Gangliosides
  • Glycolipids
  • Neuraminic Acids
  • Sulfoglycosphingolipids
  • N-glycolylneuraminic acid