Nucleotide Sequences of Alcohol Acetyltransferase Genes From Lager Brewing Yeast, Saccharomyces Carlsbergensis

Yeast. 1996 May;12(6):593-8. doi: 10.1002/(SICI)1097-0061(199605)12:6%3C593::AID-YEA593%3E3.0.CO;2-B.


The nucleotide sequences of alcohol acetyltransferase genes isolated from lager brewing yeast, Saccharomyces carlsbergensis have been determined. S. carlsbergensis has one ATF1 gene and another homologous gene, the Lg-ATF1 gene. There was a high degree of homology between the amino acid sequences deduced for the ATF1 protein and the Lg-ATF1 protein (75.7%), but the N-terminal region has a relatively low degree of homology. Southern analysis and contour-clamped homogeneous electric field analysis of Saccharomyces strains suggest that the ATF1 gene is located on chromosome XV in S. cerevisiae and that the Lg-ATF1 gene might originate from the "non-S. cerevisiae' genome of S. carlsbergensis, which is similar to that of S. bayanus and S. pastorianus.

MeSH terms

  • Acetyltransferases / genetics*
  • Amino Acid Sequence
  • Base Sequence
  • Blotting, Southern
  • Chromosome Mapping
  • Molecular Sequence Data
  • Proteins*
  • Saccharomyces / enzymology
  • Saccharomyces / genetics*
  • Sequence Homology


  • Proteins
  • Acetyltransferases
  • alcohol O-acetyltransferase

Associated data

  • GENBANK/D63449
  • GENBANK/D63450