Elevated cytosolic free Ca2+ concentrations and massive Ca2+ accumulation within vacuoles, in yeast mutant lacking PMR1, a homolog of Ca2+ -ATPase

FEBS Lett. 1996 Aug 26;392(2):194-200. doi: 10.1016/0014-5793(96)00799-5.


The Ca2+ -ATPase homolog of Saccharomyces cerevisiae, PMR1, cloned by Rudolph et al. (Cell 58 (1989) 133-145) is required for normal Golgi functions. We have investigated the role of Pmr1-protein in maintaining homeostasis of cytosolic free Ca2+ concentration ([Ca2+]i). It was found that exposure to moderately high Ca2+ concentrations led to elevated levels of [Ca2+]i in cells of pmr1 null mutant, in comparison with cells of pmr2 isogenic mutant (defective in cell-membrane Na+ - ATPase) and of an isogenic wild type. In addition, we showed that PMR1 deletion causes massive accumulation of Ca2+ in the vacuoles and affects the rates of Ca2+ influx and efflux.

MeSH terms

  • Calcium / antagonists & inhibitors
  • Calcium / metabolism*
  • Calcium-Transporting ATPases / genetics
  • Calcium-Transporting ATPases / metabolism*
  • Cytosol / metabolism*
  • Ion Transport
  • Magnesium / metabolism
  • Mutation
  • Saccharomyces cerevisiae / enzymology
  • Saccharomyces cerevisiae / genetics*
  • Saccharomyces cerevisiae / metabolism
  • Vacuoles / metabolism*


  • Calcium-Transporting ATPases
  • Magnesium
  • Calcium