bcl-1 rearrangement and cyclin D1 protein expression in mantle cell lymphoma

J Pathol. 1996 Jul;179(3):238-42. doi: 10.1002/(SICI)1096-9896(199607)179:3<238::AID-PATH566>3.0.CO;2-W.


Centrocytic lymphoma, or mantle cell lymphoma (MCL), is characterized by a chromosomal translocation t(11;14) (q13;q32) involving the bcl-1 locus on chromosome 11. Cyclin D1 is a cell-cycle regulatory protein essential for G1-S transition and has been identified as a potential transforming gene affected by the translocation. In this study, 32 cases of MCL were analysed for the bcl-1 rearrangement and cyclin D1 protein expression. In 17 cases, a rearrangement at the major translocation cluster of bcl-1 could be detected. Twenty-four cases exhibited nuclear cyclin D1 expression that was not detectable in other B-cell lymphomas (n = 40) or in normal B-cells. In nine MCL samples, cyclin D1 was expressed without a detectable bcl-1 rearrangement. The detection of a t(11;14) by means of classical cytogenetics in one of these cases, however, may suggest that this discrepancy could be due to chromosomal breakages outside the typical translocation cluster region. In two cases, a bcl-1 rearrangement was not accompanied by cyclin D1 expression. This study provides further evidence that cyclin D1 is involved in the pathogenesis of MCL and can be exploited as a diagnostic marker in the differential diagnosis of B-cell lymphomas and in the identification of MCL.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Base Sequence
  • Blotting, Southern
  • Chromosomes, Human, Pair 11
  • Chromosomes, Human, Pair 14
  • Cyclin D1
  • Cyclins / metabolism*
  • Humans
  • Immunoenzyme Techniques
  • Lymphoma, Non-Hodgkin / genetics*
  • Lymphoma, Non-Hodgkin / metabolism
  • Molecular Sequence Data
  • Neoplasm Proteins / metabolism*
  • Oncogene Proteins / metabolism*
  • Polymerase Chain Reaction
  • Proto-Oncogene Proteins / genetics*
  • Translocation, Genetic


  • Cyclins
  • Neoplasm Proteins
  • Oncogene Proteins
  • Proto-Oncogene Proteins
  • Cyclin D1