Structural and serological characterisation of two O-specific polysaccharides of Acinetobacter

Eur J Biochem. 1996 Aug 1;239(3):602-10. doi: 10.1111/j.1432-1033.1996.0602u.x.

Abstract

Extraction of dry bacteria of Acinetobacter strain 34 (DNA group 2) or Acinetobacter strain 108 (DNA group 13) by phenol/water yielded a polymer that was identified by means of serological studies and fatty acid analysis as S-form lipopolysaccharide. Degradation of the lipopolysaccharides of strains 34 and 108 in 1% acetic acid and 5% acetic acid, respectively, and gel-permeation chromatography gave the respective O-antigenic polysaccharides, the structures of which were determined, by compositional analysis and NMR spectroscopy of the polysaccharide, as [Sequence: see text] for strain 108, where D-Fucp3NBuOH represents 3-[(R)-3-hydroxybutyramido] -3,6-dideoxy-D-galactose and D-GalpANAc represents 2-acetamido-2-deoxy-D-galacturonic acid. Both structures were specifically recognised in Western blots by polyclonal rabbit antisera and there was no cross-reaction between these two structures.

Publication types

  • Comparative Study

MeSH terms

  • Acinetobacter / chemistry*
  • Acinetobacter / classification
  • Acinetobacter / immunology
  • Antibodies, Bacterial / immunology
  • Blotting, Western
  • Carbohydrate Sequence
  • Magnetic Resonance Spectroscopy
  • Molecular Sequence Data
  • O Antigens / chemistry*
  • O Antigens / immunology
  • Sequence Analysis
  • Serology
  • Species Specificity

Substances

  • Antibodies, Bacterial
  • O Antigens