Electrophysiological effects of nitric oxide in mouse superior mesenteric ganglion

Am J Physiol. 1996 Feb;270(2 Pt 1):G324-31. doi: 10.1152/ajpgi.1996.270.2.G324.

Abstract

Effects of nitric oxide (NO) generated from sodium nitroprusside (SNP) on neurons of mouse superior mesenteric ganglion (SMG) were studied in vitro using intracellular recording techniques. SNP solutions caused a membrane hyperpolarization in the majority (64%) of the neurons tested or a hyperpolarization followed by a depolarization in 8% of the neurons tested. The SNP-induced hyperpolarization persisted in a low-Ca2+ high-Mg2+ solution, indicating a direct effect of NO on the postsynaptic membrane. The hyperpolarizing effect of SNP was reduced or abolished by oxyhemoglobin. Electrical stimulation of the colonic nerves evoked a late slow excitatory postsynaptic potential (late sEPSP) in a population of neurons in normal Krebs solution. The amplitude of the late sEPSP was significantly enhanced in the presence of NG-nitroL-arginine, a NO synthase inhibitor. The results, particularly those observed with the NO synthase inhibitor, suggest that endogenous NO was released in the mouse SMG by repetitive nerve stimulation and that it modulated slow synaptic transmission, presumably by a direct action on ganglionic neurons.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Electrophysiology
  • Ganglia, Sympathetic / cytology
  • Ganglia, Sympathetic / drug effects
  • Ganglia, Sympathetic / physiology*
  • Male
  • Mesentery / innervation
  • Mice
  • Mice, Inbred Strains
  • NADPH Dehydrogenase / metabolism
  • Neurons / drug effects
  • Nitric Oxide / physiology*
  • Nitric Oxide Synthase / antagonists & inhibitors
  • Nitroarginine / pharmacology
  • Nitroprusside / pharmacology
  • Oxyhemoglobins / pharmacology
  • Synaptic Transmission / drug effects

Substances

  • Oxyhemoglobins
  • Nitroprusside
  • Nitroarginine
  • Nitric Oxide
  • Nitric Oxide Synthase
  • NADPH Dehydrogenase