H(+)-K(+)-ATPase in rabbit cortical collecting duct B-type intercalated cell

Am J Physiol. 1996 Mar;270(3 Pt 2):F518-30. doi: 10.1152/ajprenal.1996.270.3.F518.

Abstract

The role of H(+)-K(+)-adenosinetriphosphatase (H(+)-K(+)-ATPase) in the cortical collecting duct (CCD) B-type intercalated cell (B cell) is unclear. This study examined whether H(+)-K(+)-ATPase contributes to B cell intracellular pH (pHi) regulation and, if so, whether it is present at the apical or basolateral membrane. B cell Na(+)-independent pHi recovery from an acid load was only partially inhibited by peritubular N-ethylmaleimide (NEM). Complete inhibition required combining peritubular NEM either with luminal Sch-28080 or with luminal K+ removal. In contrast, neither peritubular Sch-28080 nor peritubular K+ removal altered pHi regulation. Tomato lectin, which binds to the gastric H(+)-K(+)-ATPase beta-subunit, labeled the B cell apical membrane. We conclude that the rabbit CCD B cell possesses an apical H(+)-K(+)-ATPase that plays an important role in pHi recovery from an in vitro acid load.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Cell Membrane / enzymology
  • Female
  • H(+)-K(+)-Exchanging ATPase / analysis*
  • H(+)-K(+)-Exchanging ATPase / metabolism
  • Hydrogen-Ion Concentration
  • Kidney Cortex / enzymology*
  • Kidney Tubules, Collecting / enzymology*
  • Rabbits

Substances

  • H(+)-K(+)-Exchanging ATPase