Suc1-associated Neurotrophic Factor Target (SNT) Protein Is a Major FGF-stimulated Tyrosine Phosphorylated 90-kDa Protein Which Binds to the SH2 Domain of GRB2

Biochem Biophys Res Commun. 1996 Aug 23;225(3):1021-6. doi: 10.1006/bbrc.1996.1288.

Abstract

In Swiss 3T3 fibroblasts, basic fibroblast growth factor (bFGF) stimulates the unique tyrosine phosphorylation of a protein complex around 90 kDa, as ascertained by high resolution 2-D PAGE and anti-phosphotyrosine blotting. The majority of this complex consists of the protein(s) designated previously as SNT (suc1-associated neurotrophic factor target). Tyrosine phosphorylated SNT binds to both p13suc1 protein and the SH2 domain of Grb2. Binding of SNT to Grb2 is likely to be mediated through the consensus binding motif, pYXN, on SNT. The binding of SNT to p13suc1 is independent of the pYXN motif. Tyrosine phosphorylated SNT is localised in the plasma membrane where it could form a complex with Grb2 and Sos, enabling the initiation of a novel FGF-specific signalling pathway.

MeSH terms

  • 3T3 Cells
  • Adaptor Proteins, Signal Transducing*
  • Amino Acid Sequence
  • Animals
  • Binding Sites
  • Cell Cycle Proteins*
  • Fibroblast Growth Factor 2 / metabolism*
  • Fungal Proteins / metabolism*
  • GRB2 Adaptor Protein
  • Mice
  • Molecular Sequence Data
  • Molecular Weight
  • Nerve Growth Factors
  • Nerve Tissue Proteins / metabolism*
  • Phosphoproteins / chemistry
  • Phosphoproteins / genetics
  • Phosphoproteins / metabolism*
  • Phosphorylation
  • Proteins / chemistry
  • Proteins / metabolism*
  • Schizosaccharomyces pombe Proteins*
  • Tyrosine / metabolism
  • src Homology Domains

Substances

  • Adaptor Proteins, Signal Transducing
  • Cell Cycle Proteins
  • Fungal Proteins
  • GRB2 Adaptor Protein
  • Grb2 protein, mouse
  • Nerve Growth Factors
  • Nerve Tissue Proteins
  • Phosphoproteins
  • Proteins
  • Schizosaccharomyces pombe Proteins
  • Suc1 protein, S pombe
  • Fibroblast Growth Factor 2
  • Tyrosine