Coordinate expression of nuclear and mitochondrial genes involved in energy production in carcinoma and oncocytoma

Biochim Biophys Acta. 1996 Aug 23;1316(3):203-9. doi: 10.1016/0925-4439(96)00026-9.


The expression of mitochondrial and nuclear genes involved in ATP production was examined in renal carcinomas, renal oncocytomas, and a salivary oncocytoma. Renal carcinomas were found to have a reduced mitochondrial DNA (mtDNA) content while oncocytomas had increased mtDNA contents. This parallels morphological changes in mitochondrial number in these tumours. In the carcinomas, mtDNA transcripts were decreased 5- to 10-fold relative to control kidneys, suggesting that mitochondrial transcript levels depend on the mtDNA content. In renal oncocytomas, mtDNA transcripts were slightly reduced in spite of a high mtDNA content. However, in the salivary gland oncocytoma, mtDNA transcripts were increased more than 10-fold in parallel with a 10-fold increase in mtDNA content. The expression of the nuclear DNA oxidative phosphorylation genes, ATPsyn beta and ANT2, was reduced up to 4-fold in renal carcinoma. In contrast, the levels of these two nuclear gene transcripts were induced about 4-fold in renal oncocytoma and up to 30-fold in salivary gland oncocytoma. Moreover, the ANT2 precursors were observed to change in oncocytomas. These data suggest a coordinated regulation of nuclear and mitochondrial gene expression in renal carcinomas and the specific induction of nuclear OXPHOS gene expression in oncocytomas.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Adenoma, Oxyphilic / metabolism*
  • Adult
  • Aged
  • Base Sequence
  • Carcinoma / metabolism*
  • Cell Nucleus / physiology
  • DNA Primers / chemistry
  • DNA, Mitochondrial / physiology
  • Female
  • Gene Expression Regulation, Neoplastic*
  • Glyceraldehyde-3-Phosphate Dehydrogenases / genetics
  • Humans
  • Male
  • Middle Aged
  • Mitochondria / physiology
  • Mitochondrial ADP, ATP Translocases / genetics
  • Molecular Sequence Data
  • Proton-Translocating ATPases / genetics


  • DNA Primers
  • DNA, Mitochondrial
  • Mitochondrial ADP, ATP Translocases
  • Glyceraldehyde-3-Phosphate Dehydrogenases
  • Proton-Translocating ATPases