Abstract
The purine nucleoside phosphorylase (Pu-NPase) and the pyrimidine nucleoside phosphorylase (Py-NPase) have been purified from Bacillus stearothermophilus TH 6-2. The Pu-NPase is a trimer of 30-kDa subunits and the Py-NPase is a dimer of 46-kDa subunits. The isoelectric points of Pu-NPase and Py-NPase were pH 4.3 and 4.6, respectively. The Pu-NPase could catalyze the phosphorolysis of inosine and guanosine, but not adenosine. the Py-NPase could phosphorolyze both uridine and thymidine.
MeSH terms
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Amino Acid Sequence
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Chromatography, DEAE-Cellulose
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Electrophoresis, Polyacrylamide Gel
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Geobacillus stearothermophilus / enzymology*
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Hydrogen-Ion Concentration
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Isoelectric Focusing
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Molecular Sequence Data
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Pentosyltransferases / chemistry
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Pentosyltransferases / isolation & purification*
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Purine-Nucleoside Phosphorylase / chemistry
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Purine-Nucleoside Phosphorylase / isolation & purification*
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Pyrimidine Phosphorylases
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Substrate Specificity
Substances
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Pentosyltransferases
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Pyrimidine Phosphorylases
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Purine-Nucleoside Phosphorylase