Promoting phorbol ester-TPA enhances migration of C3H 10T1/2 cells. The role of protein kinase C and its relation to carcinogenesis

Folia Biol (Krakow). 1995;43(3-4):83-7.

Abstract

Treatment of 10T1/2 cells with promoting phorbol ester drastically enhanced migration of the studied fibroblasts in a serum-supplemented medium. The same cells when exposed to ionomycin or TPA in a serum-free medium did not show any migration. The addition of 1% of serum induced spontaneous and TPA stimulated migration. Also EGF and PDGF separately or together induced the migration of 10T1/2 cells. Parallel studies of protein kinase C documented low enzymatic activity after treatment with TPA, whereas transcripts of PKC were shown independently of TPA treatment.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Carcinogens / pharmacology*
  • Cell Movement / drug effects*
  • Cell Movement / physiology
  • Cells, Cultured / drug effects
  • Cells, Cultured / physiology
  • Fetus / drug effects
  • Fetus / enzymology
  • Fibroblasts / drug effects*
  • Fibroblasts / enzymology
  • Mice
  • Mice, Inbred C3H
  • Polymerase Chain Reaction
  • Protein Kinase C / analysis
  • Protein Kinase C / biosynthesis*
  • Tetradecanoylphorbol Acetate / pharmacology*

Substances

  • Carcinogens
  • Protein Kinase C
  • Tetradecanoylphorbol Acetate