DNA immunization induces protective immunity against B-cell lymphoma

Nat Med. 1996 Sep;2(9):1038-41. doi: 10.1038/nm0996-1038.

Abstract

Idiotypic determinants of the immunoglobulin expressed on the surface of B-cell lymphomas are tumor-specific antigens (TSAs), which can be targeted by immunotherapy. Immunization with DNA constructs encoding the idiotype (ld) of a murine B-cell lymphoma induced specific anti-ld antibody responses and protected mice against tumor challenge. Use of DNA encoding an ld/GM-CSF (idiotype/granulocyte-macrophage colony-stimulating factor) fusion protein improved vaccine efficacy, and xenogeneic immunoglobulin constant region determinants were required for immunogenicity. These results indicate that DNA may be a simple and efficacious means of inducing immune responses against a weak, otherwise unrecognized tumor antigen, provided that additional stimuli are included with the DNA.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Antigens, Neoplasm / genetics*
  • Antigens, Neoplasm / immunology
  • DNA / administration & dosage
  • DNA / immunology*
  • Disease Models, Animal
  • Granulocyte-Macrophage Colony-Stimulating Factor / genetics
  • Granulocyte-Macrophage Colony-Stimulating Factor / immunology
  • Humans
  • Immunoglobulin Idiotypes / genetics
  • Immunoglobulin Idiotypes / immunology
  • Lymphoma, B-Cell / immunology
  • Lymphoma, B-Cell / prevention & control*
  • Mice
  • Mice, Inbred C3H
  • Recombinant Fusion Proteins / genetics
  • Recombinant Fusion Proteins / immunology
  • Tumor Cells, Cultured
  • Vaccines / administration & dosage
  • Vaccines / immunology*

Substances

  • Antigens, Neoplasm
  • Immunoglobulin Idiotypes
  • Recombinant Fusion Proteins
  • Vaccines
  • Granulocyte-Macrophage Colony-Stimulating Factor
  • DNA