Modulation of growth and apoptosis response in PC-3 and LNCAP prostate-cancer cell lines by Fas

Int J Cancer. 1996 Sep 4;67(5):709-14. doi: 10.1002/(SICI)1097-0215(19960904)67:5<709::AID-IJC20>3.0.CO;2-Z.


Fas/APO-1 is a cell-surface protein, a member of the TNF-receptor family, and it potentially induces apoptosis. In presence of an apoptosis-inducible anti-human Fas MAb, Fas-negative control PC-3 human prostate-cancer cells did not undergo morphological changes, while PC-3 human Fas transfectants showed apoptotic changes in vitro. However, LNCaP human Fas transfectants, as well as Fas-negative control LNCaP human prostate-cancer cells, were Fas-resistant. The growth of Fas-transfected PC-3 tumor was retarded compared with that of control PC-3 tumor in vivo without stimulation of anti-human Fas MAb. Anti-human Fas MAb administration in vivo caused macroscopic Fas-transfected PC-3 tumors formed in BALB/c nude mice to undergo apoptosis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adaptor Proteins, Signal Transducing
  • Animals
  • Antibodies, Monoclonal / pharmacology
  • Apoptosis / drug effects*
  • Apoptosis Regulatory Proteins
  • Carrier Proteins / genetics
  • Carrier Proteins / immunology
  • Carrier Proteins / pharmacology*
  • Cell Division / drug effects*
  • DNA, Complementary / genetics
  • Humans
  • Intracellular Signaling Peptides and Proteins
  • Male
  • Mice
  • Mice, Inbred BALB C
  • Mice, Nude
  • Microscopy, Electron
  • Prostatic Neoplasms / pathology*
  • Transfection
  • Tumor Cells, Cultured


  • Adaptor Proteins, Signal Transducing
  • Antibodies, Monoclonal
  • Apoptosis Regulatory Proteins
  • Carrier Proteins
  • DNA, Complementary
  • FAF1 protein, human
  • Faf1 protein, mouse
  • Intracellular Signaling Peptides and Proteins