Prolonged induction of c-fos in neuropeptide Y- and somatostatin-immunoreactive neurons of the rat dentate gyrus after electroconvulsive stimulation

Brain Res. 1996 May 13;720(1-2):111-9. doi: 10.1016/0006-8993(96)00158-8.


Induction of c-fos mRNA and Fos was studied in the hilus and granular layer of the dentate gyrus at various times up to 24 h after single electroconvulsive stimulation (ECS) using in situ hybridization and immunocytochemistry. In both areas of the dentate gyrus, a prominent induction of c-fos mRNA and Fos was observed. Compared to the granular layer, however, c-fos mRNA and Fos in hilar cells reached maximum later and remained elevated considerably longer. Several neurochemically distinct populations of hilar neurons have been described, some of which contain neuropeptide Y (NPY) and/or somatostatin (SS). Using double-labelling immunocytochemistry, we examined to what extent Fos was induced in these hilar neurons after ECS. Although a minor population of non-NPY non-SS cells displayed Fos induction early after ECS, prolonged induction of Fos almost exclusively occurred in NPY or SS neurons. The Fos-immunoreactive NPY or SS neurons only amounted to about 50% of the total hilar population of NPY or SS neurons. The present observations suggest that a subpopulation of hilar NPY and SS neurons may be central to the actions of electroconvulsive seizures in the dentate gyrus.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Dentate Gyrus / cytology
  • Dentate Gyrus / metabolism
  • Dentate Gyrus / physiology*
  • Electric Stimulation
  • Immunohistochemistry
  • In Situ Hybridization
  • Male
  • Neurons / metabolism
  • Neurons / physiology*
  • Neuropeptide Y / metabolism*
  • Oligonucleotide Probes
  • Proto-Oncogene Proteins c-fos / biosynthesis*
  • RNA, Messenger / biosynthesis
  • Rats
  • Rats, Wistar
  • Seizures / metabolism*
  • Somatostatin / metabolism*


  • Neuropeptide Y
  • Oligonucleotide Probes
  • Proto-Oncogene Proteins c-fos
  • RNA, Messenger
  • Somatostatin