Differential expression of vasoactive intestinal peptide receptors 1 and 2 (VIP-R1 and VIP-R2) mRNA in murine lymphocytes

J Neuroimmunol. 1996 Aug;68(1-2):27-38. doi: 10.1016/0165-5728(96)00063-x.


Vasoactive intestinal peptide (VIP), a neuropeptide present in the lymphoid microenvironment, modulates cytokine expression and affects T cell proliferation. Recent molecular studies identified two VIP receptors. VIP-R1 and VIP-R2, primarily in nonlymphoid cells. In this study, we investigate the expression of VIP-R1 and VIP-R2 mRNA in unstimulated and stimulated lymphocytes and thymocytes, and in various lymphocyte subpopulations. In contrast to VIP-R1 which is constitutively expressed, the expression of VIP-R2 is induced only following stimulation through the TCR-associated CD3 complex. Both CD4+ and CD8+ T cells express VIP-R1 and VIP-R2. Two T cell lines, EL-4.IL-2 and D10.G4.1 express exclusively VIP-R2. VIP induces the expression of the VIP-R2 gene in the absence of additional stimuli. Differential expression and regulation of the two VIP receptors in T lymphocytes suggests different physiological roles in mediating the immunomodulatory activities of VIP and related neuropeptides.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • B-Lymphocytes / drug effects
  • B-Lymphocytes / physiology
  • Base Sequence
  • Cell Line / chemistry
  • Cell Line / immunology
  • Female
  • Gene Expression / immunology
  • Lipopolysaccharides / pharmacology
  • Lymphocyte Activation / genetics
  • Lymphocyte Activation / immunology
  • Lymphocyte Subsets / physiology
  • Mice
  • Mice, Inbred BALB C
  • Molecular Sequence Data
  • RNA, Messenger / metabolism
  • Receptors, Vasoactive Intestinal Peptide / genetics*
  • Receptors, Vasoactive Intestinal Peptide / immunology*
  • Receptors, Vasoactive Intestinal Peptide, Type II
  • Sequence Analysis, DNA
  • Spleen / cytology
  • T-Lymphocytes / chemistry*
  • T-Lymphocytes / physiology*
  • Thymus Gland / cytology
  • Time Factors


  • Lipopolysaccharides
  • RNA, Messenger
  • Receptors, Vasoactive Intestinal Peptide
  • Receptors, Vasoactive Intestinal Peptide, Type II
  • Vipr2 protein, mouse

Associated data

  • GENBANK/S82966