Ascorbic acid preferentially enhances type I and III collagen gene transcription in human skin fibroblasts

J Dermatol Sci. 1996 Mar;11(3):250-3. doi: 10.1016/0923-1811(95)00640-0.


Ascorbic acid is a potent stimulator for type I and III collagen expression in human skin fibroblasts; stimulation of type I and III collagen synthesis and their mRNA levels by ascorbic acid has been reported previously. Nuclear run-on experiments demonstrated that ascorbic acid enhanced the transcription of type I and III collagen genes 4- and 3.4-fold respectively, whereas transcription of type IV collagen was slightly stimulated (1.7-fold). The results suggest that ascorbic acid preferentially enhanced type I and III collagen transcription.

MeSH terms

  • Ascorbic Acid / pharmacology*
  • Cells, Cultured
  • Collagen / drug effects*
  • Collagen / genetics*
  • Fibroblasts / drug effects
  • Fibroblasts / metabolism
  • Humans
  • RNA, Messenger / analysis
  • Skin / drug effects*
  • Skin / metabolism
  • Transcription, Genetic / drug effects*


  • RNA, Messenger
  • Collagen
  • Ascorbic Acid