A T-T hybridoma (F6.6.2) which secretes a T-suppressor factor (TsF) specific for cryptococcal capsular polysaccharide (glucuronoxylomannan, GXM) was tested to determine if antigen-presenting cells (APC) were necessary for activation of the hybridoma to secrete TsF. Normal, syngeneic spleen cells were required along with GXM before TsF could be detected in culture supernatants. Ts cells did not secrete TsF unless the APC were obtained from mice which were identical at the "so-called' I-J sublocus as defined by the difference between B10.A(3R) and B10.A(5R) mice. The APC was adherent and could be depleted from spleen cell suspensions by treatment with anti-I-J and complement but not anti-I-A and complement. Additionally, treatment with anti-T cell serum or anti-immunoglobulin and complement did not remove the APC function of the spleen cell population. A role for I-E antigens in the function of the APC was determined by blocking antigen presentation to the suppressor cell with anti-I-E antibodies. The polysaccharide was associated with splenic adherent cells as extensive washing of the APC after incubation with GXM did not eliminate the antigen presenting function of the population.