Cloning, in vitro expression, and tissue distribution of a human prostaglandin transporter cDNA(hPGT)

J Clin Invest. 1996 Sep 1;98(5):1142-9. doi: 10.1172/JCI118897.


We recently identified a cDNA in the rat that encodes a broadly expressed PG transporter (PGT). Because PGs play diverse and important roles in human health and disease, we cloned human PGT (hPGT) from an adult human kidney cDNA library. A consensus sequence (4.0 kb) derived from several clones, plus 3' polymerase chain reaction amplification, exhibited 74% nucleic acid identity and 82% amino acid identity compared to rat PGT. When transiently expressed in HeLa cells, a full-length clone catalyzed the transport of PGE1, PGE2, PGD2, PGF2alpha, and, to a lesser degree, TXB2. Northern blotting revealed mRNA transcripts of many different sizes in adult human heart, placenta, brain, lung, liver, skeletal muscle, pancreas, kidney, spleen, prostate, ovary, small intestine, and colon. hPGT mRNAs are also strongly expressed in human fetal brain, lung, liver, and kidney. The broad tissue distribution and substrate profile of hPGT suggest a role in the transport and/or metabolic clearance of PGs in diverse human tissues.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Antiporters / biosynthesis
  • Antiporters / genetics*
  • Biological Transport
  • Cloning, Molecular
  • Consensus Sequence
  • DNA, Complementary
  • DNA-Binding Proteins / biosynthesis
  • DNA-Binding Proteins / genetics*
  • Fetus
  • Gene Library
  • Humans
  • Molecular Sequence Data
  • Organic Anion Transporters
  • Prostaglandins / metabolism*
  • Rats
  • Recombinant Proteins / biosynthesis
  • Sequence Analysis, DNA
  • Sequence Homology, Amino Acid
  • Sequence Homology, Nucleic Acid
  • Species Specificity
  • Tissue Distribution


  • Antiporters
  • DNA, Complementary
  • DNA-Binding Proteins
  • Organic Anion Transporters
  • Prostaglandins
  • Recombinant Proteins
  • SLCO2A1 protein, human
  • Slco2a1 protein, rat

Associated data

  • GENBANK/AF056719
  • GENBANK/AF056720
  • GENBANK/AF056721
  • GENBANK/AF056722
  • GENBANK/AF056723
  • GENBANK/AF056724
  • GENBANK/AF056725
  • GENBANK/AF056726
  • GENBANK/AF056727
  • GENBANK/AF056728
  • GENBANK/AF056729
  • GENBANK/AF056730
  • GENBANK/AF056731
  • GENBANK/AF056732
  • GENBANK/U70867