Immortalisation of human urothelial cells

Urol Res. 1995;23(6):377-80. doi: 10.1007/BF00698738.


A cell line derived from the urothelium lining the ureter of a 12-year-old girl was immortalised using a temperature-sensitive SV40 large T-antigen gene construct, and designated UROtsa. Following immortalisation, UROtsa cells expressed SV40 large T-antigen, but did not acquire characteristics of neoplastic transformation, including growth in soft agar or the development of tumours in nude mice. Metaphase spreads had a normal chromosomal appearance and number. UROtsa cells remained permissive for cell growth at 39 degrees C, indicating that they did not retain temperature sensitivity. UROtsa provides an in vitro model of "normal" urothelium.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antigens, Polyomavirus Transforming
  • Cell Line, Transformed / cytology*
  • Cell Transformation, Viral
  • Child
  • Culture Media
  • Epithelial Cells
  • Female
  • Humans
  • Mice
  • Mice, Nude
  • Temperature
  • Transfection
  • Ureter / cytology*


  • Antigens, Polyomavirus Transforming
  • Culture Media