A rat model for hypoxic-ischemic brain damage in very premature infants

Biol Neonate. 1996;69(5):327-41. doi: 10.1159/000244327.


We proposed that hypoxic-ischemic injury suffered on postnatal day 1 (P1) in the newborn rat would yield significant and reproducible brain injury simulating that seen in a midgestation human brain. The P1 pup was subjected to ipsilateral common carotid ligation followed by 3.5 h of 6% O2 and demonstrated consistent histological changes in the ipsilateral hemisphere. At P2 neuronal loss and edema were pronounced in cortex and caudate; edema subsequently declined by P3. Astrocytic and microglial reactivity were seen as early as 24 h after injury, and by P10 both were extensive in damaged areas. There was marked loss of white matter on the affected side. We have demonstrated a reproducible model of hypoxic-ischemic injury to the very immature brain that can be used to elucidate mechanisms of neuronal loss and periventricular leukomalacia.

MeSH terms

  • Animals
  • Animals, Newborn*
  • Astrocytes / pathology
  • Brain Diseases / etiology*
  • Brain Edema
  • Brain Ischemia / complications*
  • Carotid Arteries / surgery
  • Disease Models, Animal*
  • Female
  • Gestational Age*
  • Humans
  • Hypoxia, Brain / complications*
  • Infant, Newborn
  • Infant, Premature
  • Ligation
  • Microglia / pathology
  • Microglia / physiology
  • Neurons / pathology
  • Pregnancy
  • Rats
  • Rats, Sprague-Dawley